14 Avril – Conférence de Monsieur ALBERTAZZI Lorenzo,
14 h Auditorium Neurocampus, Université de Bordeaux Site Carreire
Super resolution imaging of nanomaterials: looking at chemical structures at the interface with biology
Nanoscopy for Nanomedicine Group, Institute for Bioengineering of Catalonia (IBEC)
C\ Baldiri Reixac 15-21, Helix Building, 08028 Barcelona
The use of nanomaterials for biomedical applications such as drug delivery and regenerative medicine is a great challenge for synthetic chemistry and nanotechnology. A crucial factor limiting the design of effective materials is the lack of understanding about material-cell interactions that hampers the rational design of nanostructured materials.
Here we discuss the use of super resolution microscopy to image materials in vitro and in mammalian cells. This novel technique, allowing to obtain a resolution down to 20nm, had a dramatic impact in the field of cell biology, however its use in the field of chemistry and nanotechnology is poorly explored. Super resolution microscopy offers nanometric resolution and multicolor ability, therefore it is an ideal tool to study nano-sized structures in vitro and in cells. We employed Stochastic Optical Reconstruction Microscopy (STORM) to image biomaterials in vitro, with special emphasis on supramolecular polymers and nanoparticles, unveiling novel information on materials structure and dynamics, a key issue of supramolecular materials.
Moreover we propose a methodology to image nano-sized materials in cells, tracking them during their membrane targeting, cell uptake and intracellular targeting. We show how 2-color STORM can be used to perform nanometric-accurate colocalization unveiling at the molecular level materials-cell interactions. This allows to look at nanomaterials in action with new eyes and use the information obtained for the “STORM-guided” design of novel nanomaterials for a variety of biomedical applications.
References:
Multicomponent Supramolecular Polymers as a Modular Platform for Intracellular Delivery
ACS Nano, 2016, 10 (2), 1845–1852
Super Resolution Imaging of Nanoparticles Cellular Uptake and Trafficking
ACS Appl. Mater. Interfaces, 2016, 8 (10), pp 6391–6399
Probing exchange pathways in one-dimensional aggregates using super resolution microscopy
Science, 2014, 344(6183): 491-5
