• Vascular pulmonary pathophysiology
• Bronchial remodeling in obstructive pulmonary disease

• Identification of mechanisms and of molecular and cellular targets
• Clinical trials
• Development of animal models and imaging technique for functional exploration of these models
• Cardiovascular adaptation to ischemia
• Systemic angiogenesis

• Autoimmunity and skin inflammatory disorders
• Autoimmunity and pigmentation
• Phenotype and function tissue resident cells
• Impact of soluble factors on epithelial cells
• Development of new therapeutic approaches for autoimmune and inflammatory skin disorders

• Tissue cultures : isolation and cultures of skin cells (melanocytes, keratinocytes) ; extraction and culture of T cells from skin samples
• 3D cultures of human reconstructed pigmented epidermis
• T cell polarization
• Flow cytometry : cell sorting and multiparametric analyses
• Immunostaining on tissues and cells samples
• Real time PCR analyses
• Western Blot, ELISA

• Erythropoietic porphyria diseases
• Mouse models of porphyria
• Cell and gene therapy of genetic disorders
• iPSCs generation
• Hematopoietic and hepatic differentiation from iPSCs
• Development of safe iPSCs for clinical prospects

• Lentiviral vectors
• Directed homologous recombination by nucleases
• Hematopoietic differentiation and graft in NSG mice
• iPSC expertise

• Rare skin disorders
• Skin pigmentary diseases
• Skin aging and cancers
• Metabolic plasticity of skin stemness and quiescent cells
• The consequences of the oxidative and energy metabolism on cell cycle, particularly on the quiescent and the slow cycling or dormant skin cells
• Development of new therapeutic approaches for skin disorders such as xeroderma pigmentosum

• Tissue cultures : isolation and cultures of skin cells (melanocytes, fibroblasts, keratinocytes
• 3D cultures of human reconstructed epidermis and pigmented epidermis
• Mouse models and humanized skin mouse models
• Modeling of skin pathologies (using pathological cells or genetically modified cells)
• Analysis  of oxidative stress
• Analysis of DNA repair capacity
• Energy metabolism analysis
• Flow cytometry : cell sorting and multiparametric analyses
• Immunostaining on tissues and cells samples
• Real time PCR analyses
• Western Blot, ELISA

Our project revolves around two majors axis:

• to conceive, develop, and upgrade to clinical scale the procedures of ex vivo transformation of grafts based on hematopoietic stem and progenitor cells and mesenchymal stem cells (whatever sources: bone marrow, steady state peripheral blood, mobilized peripheral blood, cord blood, fat tissue…)
• the subsequent conservation of these grafts.

We study the characterization, the regulation and relationship between hematopoietic stem cells and mesenchymal stem cells in order to better understand their specific needs and control their fate ex vivo. Based on our expertise in the field of stem cells we develop tools in order to address these stem cells in terms of their functional properties and molecular signature at gene expression and energetic metabolic levels.

• Wnt/Frizzled and Hedgehog signaling pathways in coordinating metabolism, formation, maturation and remodeling of the vascular network
• Influence of platelets in endothelial cells properties
• Pharmacological and experimental approaches of drugs that may impair angiogenic pathways
• Effective organ perfusion in situation of ischemic heart hemodynamic failure in experimental model and human clinical trial.

• In vitro
  •     Cell culture (vascular cells, hematopoietic cells,etc…)
  •     In vitro models (proliferation, migration, chemotactism, cell tracking, angiogenesis, flux adhesion)
  •     Biochemistry (WB, IP, pulldown)
  •     Molecular Biology (Cloning, Promoter analysis, Double hybrid selection, transgenic mice)
  •     Histology, immunohistology
  •     Analysis (Fluorescence spectroscopy, Mass spectroscopy, infrared spectroscopy)
• In vivo
  • Transgenic mice
  • Mouse experimental models:
    • Angiogenesis (corneal angiogenesis, retina development)
    • Vessel diseases (atherosclerosis, vessel denudation, intimal thickening)
    • Ischemia (hind limb ischemia, heart infarction, brain ischemia)
    • Bonne marrow transplantation
    • Measurement of physiologic cardiovascular parameters
  • Pig experimental models:
    • Acute heart ischemia
    • Chronic heart ischemia
    • Ischemia-reperfusion
• Imaging
  • White field and fluorescent microscopy
  • Confocal microscopy
  • Time lapse microscopy
  • Rapid film imaging
  • Perfusion imaging (laser Doppler)
  • Xray tomography
  • Infrared imaging

• Clinics
  • Pharmacology
  • Clinical trials

In progress

In progress

In progress

In progress

• Developing new therapeutic approaches of neurodegenerative disorders by facilitating the translational research from bench to bedside.

• Increasing its scientific visibility and attractiveness in translational (Parkinson, Alzheimer, Obsessive compulsive disorders, Addiction) and fundamental neurosciences (Action planning, Cognition, molecular substrates of synaptic transmission) by building on the potential of its teams and the dynamics set in motion since its creation.

In progress

• Bone and vascular tissue engineering using human stem cells and 3-dimensional matrices
• Cell to cell communication for tissue regeneration
• Bioprinting and Biofabrication

• Human adult stem cell cultures (MSCs, ASCs, Endothelial progenitor cells, HUVEC..).
• Analysis of Cell - 3D matrix interactions (polymers, composite polymers, calcium phospahate…) using cell biology and molecular biology
• 3D biopabrication, 3D bioprinting assisted by Laser
• Preclinical experimental models in mice, rat , sheep and pig) (ectopic site, orthotopic sites, bone defects, vascular replacement)
• Histological analysis
• Bioimaging

• Developmental angiogenesis (neo-vascularization in the mouse retina)
• Vascular cell biology
• Pathophysiology of pulmonary hypertension (chronic hypoxia, monocrotaline, hyperoxia in animal models (TRPV4 -/-, Cx 43 +/-, Cav 3.1 -/- mice), rats and neonates)
• Airborne particulate matter and human health

• Tissue culture: primary endothelial cells and all types of cell lines, plasmid and RNAi transfection, scrape loading
  • Human and murine endothelial cells isolation
    • 3D culture in collagen gels
    • DNA and RNA extraction, qPCR, genotyping,  DNA subcloning, directed mutagenesis, DNA sequencing, southern blotting, chromatin immunoprecipitation
  • Basic biochemistry: Western Blot, immunoprecipitation
  • Pull down assay, kinase assay
    • Production and purification of recombinant proteins
    • ELISA
    • FACS: cell sorting and analysis
    • In vitro angiogenesis assay on Matrigel and aortic ring assay, Cell invasion assay and Matrix degradation assay
    • Immunostaining on cultured cells and tissues
  • Epifluorescence and confocal immunofluorescence, brightfield and fluorescence videomicroscopy
  • En face viewing of the mouse aorta
    • Image processing: ImageJ and Imaris Software
  • Cellular calcium imaging (fura2/epifluorescence, fluo-4/confocal)
    • Fluorescence imaging on living cells (mitochondrial membrane potential, mitochondrial calcium) and on isolated vessels or lung slices
    • Patch-clamp
    • Contraction on isolated vessels (organ baths, myograph, arteriograph)
    • Histology
  • Electron paramagnetic resonance to measure superoxide anion and NO (EPR)
    • Hemodynamic measurements (arterial pressure, intra-cardiac pressure)
  • Intracardiac perfusion

In progress

In progress

• Islet cell biology and signalling
• Hybrid cell-based biosensors

• Electrophysiology of the islet micro-organ
• Microfluidics of islet preparations
• Functional diabetes tests in mice
• Functional tests of islet cells

• Advanced biomaterials (supramolecular engineering) for medicine regenerative applications (Synthesis and applications of hybrid bioinspired molecules: Nucleoside Lipids, Gycosylated Nucleoside Lipids, Amino Acid nucleoside lipids etc.
• Conception, design and development of specific drug delivery systems (DDS)
• Therapeutics nucleic acids based on Lipid-oligonucleotide conjugates (LONs) for biomedical applications (cancer etc).

• Conventional facilities for molecular, supramolecular and physico chemistry (organic synthetic lab, NMR, UV-Vis, tensiometer, rheometers, nano-sizer, zeta potential, ultrasound…)
• Specific synthesis facilities: NMR, Automated oligonucleotides synthesis, microwave-assisted synthesis, etc.
• Molecular and supra molecular chemistry for Biomedical applications
• Nano sciences
• Drug delivery
• Bioinspired systems
• Nucleic acid chemistry

In progress

In progress

• Molecular modeling and molecular dynamics
• Lipidic assemblies
• Biomembrane models
• Mono- and bi-layers
• Micelles
• Membrane proteins
• GPCR
• Building of protein models by sequence homology
• Apoptotic cascade
• Kinases and drug-design
• Mesoscopic or coarse-grain dynamics

• All-atom molecular dynamics
• Drug-design & high-troughput in-silico screening
• Surface plasmon microscopy

• Synthesis of bifunctionalized materials with bioactive microdomains for Mesenchymal Stem Cell differentiation
• Impact of bioactive  nanopillars or nanopits on Mesenchymal Stem Cell differentiation
• Multi-functionalization of polymer surfaces with active principles able to favour progenitor endothelial cell adhesion and differentiation
• Synthesis of materials able to release antibiotics and to fight bone infection
• Engineered bioactive microchannels for vascularization
• Use of cell microvesicles for diagnosis and tissue engineering  

Tools of treatment and preparation of materials

• Glove boxes (JACOMEX and MECA2000) allowing to work at low pressure, high temperature (until 350°C) and at controlled atmosphere (Ar)
• Photolithography technique

Tools for physico-chemical characterization of the biomaterials and characterization of organic intermediaries of synthesis

• Contact angle measurements
• Leica Fluorescence microscope
• Spectrometer NMR 300 MHz
• Use of platforms :
  • RESEARCH INFRASTRUCTURE, Magnetic Nuclear Resonance, Very High Fields FR3050 CNRS (CBMN/IECB)
  • PLACAMAT : PLateforme Aquitaine de Caractérisation des MATériaux : UMS 3626 (SEM, TEM, Auger and XPS facilities)
  • ELORGA Plateform (photolithography)
  • ELORPRINTEC Platform (Nanoimprint)

Human cell cultures (Mesenchymal Stem Cells, Human Umbilical Vein Endothelial Cells, Endothelial Progenitor Cells, osteoblasts, pericytes) in L2 laboratory
Analysis of cell-material interactions using cell biology and molecular biology

• LEICA Fluorescence microscope  (Timelaps)
• ELX 808 Absorbance Reader
• Western-blot

In progress

Designing and synthesizing copolymers that can encode both self-assembly and bioactivity properties

• Sociology of science
• Knowledge diffusion
• Science controversy

• Observation
• Survey research
• Social statistics

• Economy of innovation
• Economy of science and technology transfert
• Economics of Pharmaceutical Industry
• Diffusion of health innovation

• Scientometrics
• Patent Mapping
• Text analysis (with Iramuteq© software)
• Network analysis (with Gephi©, Intellixir®, Vos Viewer© softwares)
• Statistics and economectrics analysis (with Xlstat® or STATA®))
• Data vizualization (with Tableau®)

• Care, ethics & health
• Nietzche and biology
• Biopolitic

• Nature of standard
• Epistemology
• Political and historicity of standards